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What does the yellow vertical line signify in the YouTube video timeline?

Is it an ad start point, or is it its you-can-play-now point as many believe?

It's not clickable nor does hovering over it do anything special. What it is there for?

References:

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  • Anyone know why they are there?
    – User
    Feb 12 '15 at 5:15
12

It marks the point when an ad pops up in the player.

Here is the marker visible in a video:

YouTube - Yellow Marker No Ad

And here is the popup advert when the player reaches the marker:

YouTube - Yellow Marker Popup Ad visible

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